Measurement of Lipid Hydroperoxides by the Ferric-Xylenol Orange Method (1) Characteristics of the Ferric-Xylenol Orange/Membrane Phosphatidylcholine Complex

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Measurement of lipid hydroperoxides by the ferric-xylenol orange method (1) characteristics of the ferric-xylenol orange/membrane phosphatidylcholine complex.

The ferric-xylenol orange (FOX) method for measurement of hydroperoxides is based on a technique that employs reduction of peroxides in an acidic condition by Fe2+ and formation of the colored ferric-xylenol orange (XO/Fe3+) product with a peak at 560 nm. The 560 nm absorbance peak of XO/Fe3+ shifts to a 610 nm peak with high absorption intensity in the presence of phosphatidylcholine. This is ...

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ژورنال

عنوان ژورنال: Journal of Nutritional Science and Vitaminology

سال: 2009

ISSN: 0301-4800,1881-7742

DOI: 10.3177/jnsv.55.9